The visible fluorescence spectra has been measured from hepatocarcinoma,hapetocirrhosis, normal human serums and serum which hepatocarcinoma tumor has been cut. The spectra profiles are substantially different. The Hepatocarcinoma serum shows two features which make the hepatocarcinoma differing from the other samples.And, the features may been thought a rule to diagnose the hepatocarcinoma. At the same time, this method is showed reasonable.