A method making multiplex holograms of speech organs was proposed.Both the line width in the 2D line drawing of speech organs and diffraction efficiency of the multiplex holograms is discussed.A bright 2D line drawing of speech organs is reconstructed from the multiplex holograms when it is illuminated with a small white light source.It is animated that 2D line drawing of speech organs comprises lips,tongue,teeth and soft palate etc when the multiplex holograms is moved slowly.The applied foreground of the multiplex hologram of speech organs is discussed finally.