The paper uses wavelet transform method to study the fluorescence spectrum of mice's fibrosis serum,whose character could be successfully distilled using mexh wavelet.The characteristic peaks could be gotten in 508,527,556,589 and 633 nm,and the ratio of the intensity of first three peak to that of the fourth could differentiate the healthy and the fibrosis in certain degree.It proves that wavelet transform could be a valuable method to analyze fluorescence spectrum.