Adenosine receptors have been further subdivided into four subtypes,A₁,A2A,A2B and A 3,all of which couple to G proteins and have been cloned successfully.In order to investigate which adenosine receptors are inv olved in adenosine-induced cellular Ca²⁺ dynamics in dorsal root ganglion (DRG) ne urons and whether the effects of adenosine on intracellular Ca²⁺ dynamics are dose-response,in this study we use selective agonists and antagonists of adenosine receptors by laser scanning confocal micro scopy and Ca²⁺ fluorescent dye Fluo 4/AM.Our results show that using adenosine A_1and A_3receptors selective antagonists could block adenosine-induced Ca²⁺ dynamics,which is not fou nd with adenosine A2A and A2B receptors selective antagonists.It shows that adenosine A_1receptor (A₁R) and adenosine A_3receptor (A₃R) might be related to adenosine-evoked Ca²⁺ release in DRG neurons.In addition,we find that the adenosine stimulation of intracellular Ca²⁺ concentration in DRG neurons is dose-response.This study suggests that the cellular ion signaling could be detected efficiently by laser scanning confocal microscopy.The results are helpful to reveal the role of adenosine and adenosine receptor in ion signal transmission during pain.