Fluorescence spectral imaging is used to detect pilose antler pieces in this paper,in order to provide a fast and non-destructive method for the identification of pilose antl er.Pilose antler standard is supplied by Guangdong Provincial Institute for drug control and 12pilose an tler samples from different market sources are analyzed by the fluorescence spectral imaging emplo ying the crystal liquid spectral imaging instrument.The spectral characteristic curves of pilose antler standard and 12samples are extracted from their spectral cubes,and the Euclid ean clustering analysis method is used to analyze the curves for the identification of pilose antler samples.In t he fluorescence spectral imaging system,the center wavelength of narrow band ultraviolet light source is 254nm,the spectral resolution is 5nm,the spectral range is from 410nm to 670nm,and the spatial r e solution is 4000×4000. The testing results of the pilose antler samples using spectral imaging are cons istent with the traditional characteristics and microscopic identification results.It shows that the fluorescence spectral imaging method can be used to identify the authenticity of pilose antler,and it is simpler and more objective.