In order to improve the reliability of food pathogen detecti on,a system based on (adenosine 5′-triphosphate (ATP) bioluminescence reactio n kinetics data fitting and optic calibration is developed. An LED with the same wavelength as ATP bioluminescence is used to simulate the light absorption and scattering of the food samples.By this method,the food col or and turbidity interference are reduced.A photomultiplier tube (PMT) is used to record ATP intensity data con tinuously.Based on bioluminescence reaction kinetics equation,the bioluminescenc e intensity data is fitted to reduce the system noise and improve the measuring range.Optic calibration algorithm b ased on LED is presented,and data fitting algorithm based on ATP bioluminescence kinetics is studied.A pathogen detection system based on bioluminescence and optic calibration is studied and dev eloped in this work.S.aureus(ATCC26001) samples are detected and the results sho w that the system has a good linearity with the traditional culture counting method with correlation coeffici ent of 0.98.The detection range of pathogens is from 10²CFU/mL to 10⁸CFU/m L.The reproducibility of the luminometer is tested and the coefficient variation is within 5.05%.This work may provide a new strategy for ATP bioluminescence used in food hygiene and safety.