In order to enhance the efficiency of data acquisition of Fourier ptyc hographic microscopy as well as acquire a high-resolution color image,the wavele ngth multiplexed Fourier ptychographic microscopy (WMFPM) was proposed.However,there is no clear guideline when one choses the parameters of the experiment system.In order to investigate the impa ct of some key parameters of this technique on recovering quality,simulation experiments are c arried out to study the influence of spatial sampling ratio,spectral overlap ratio and the c ombination of the two on the quality of the final recovery.The research results show that spatial sampling ratio must satisfy the requirement of spatial sampling under the same s pectral overlap ratio.As a result,choosing the right size of the camera pixel to meet the spat ial sampling requirements is of vital importance.On the other hand,under the same spatial sampling ratio,the spectral overlap ratio range of 50%－70% is the best to recov er a high-resolution color image.Therefore,the appropriate distance between the LED and the sample should be selected according to the actual experimental conditions.The research results provide a certain guiding role for the design of system and the selection of the optimal system parameters .