Nasopharyngeal carcinoma (NPC) is one of the head and neck cancers wit h strong metastasis feature,and EBV-encoded latent membrane protein 1(LMP1) is consider ed to closely connect with development,invasion and metastasis of NPC.But the mechanisms of proliferation and metastasis of NPC induced by LMP1are not clear at present.Research progres s on the mechanisms of proliferation and metastasis of NPC are briefly presented in this article.As an effective technology,various fluorescence detection technologies have been w idely applied in life sciences.Characteristics of those technologies (e.g,ImmunoFluorescence ,Bimolecular Fluorescence Complementation and Fluorescence Resonance Energy Transfer) which a re applied in those researches on the mechanisms of proliferation and metastasis of NPC are de tailed summarized.Furthermore advantages and disadvantages of those technologies are a nalyzed, respectively.According to closely connection between the cytoplasmic membrane a nd proliferation and metastasis of NPC,the Possibilities that utilizing the techno logy combining with total internal reflection fluorescence and Fluorescence Resonance Energy Tr ansfer to solve the problem about the mechanisms of proliferation and metastasis of NPC th rough high resolution imaging of LMP1on cytoplasmic membrane of NPC cells are analyzed.