Tunability is one of the important indicators of multi-wavelength fib er lasers and can be used to evaluate laser performance.In order to enhance the tunability of multi-wavelen gth fiber lasers to meet demand for dense wavelength division multiplexing systems and microwave photonics,based on the Brillouin effect,a multi-wavelength Brillouin erbium-doped fiber laser (MWBEFL) with 1×5tunabl e Brillouin frequency spacing is designed by combining three Brillouin frequency shift (BFS,Including two double Brillouin frequency shift cavity and one single Brillouin frequency shift cavity) cavities and three erbium-dope d fiber amplifiers (EDFA).EDFA is used as a switch for stimulated Brillouin scattering (SBS) to dominate multi-wa velength generation with different frequency spacing.By properly adjusting the output power of the different EDFAs .The multi-wavelength outputs with the single,double,triple,quadruple,and quintuple Brillouin frequency sp acing are obtained in the experiment, and the wavelength spacing are 0.086nm,0.172nm,0.258nm,0.344nm,and 0.430nm,respectively,the corresponding number of Stokes light (BS) is 18,7,5,2,1,respectively,and t he output laser shows good power stability during the measurement time of 30mins,the maximum power fluctuations are 0.867dB,0.594dB, 0.438dB,0.814dB,0.389dB.