The mechanism of viewing autostereoscopic 3D display is contradictory to human visual physiology. Prolonged viewing time could cause headache,eye distension,double vision and o ther symptoms of visual fatigue. It is essential to choose the appropriate physiological indicators for objective evaluation so as to prevent and eliminate visual fatigue.The present research investigates the effect of viewin g autostereoscopic 3D display on binocular visual function parameters.24subjects are selected through visual ex amination.Subjects are randomly divided into two groups by whether watching 2D video or 3D video in 30minutes. Objective evaluation indicators such as binocular fusion range,AC/A and CFF are measured before and after viewi ng.The subjects also complete subjective evaluation of visual fatigue and VIMS.The experiment verifies fusion range,AC/A and CFF decrease as the degree of visual fatigue and VIMS increases.With respect to fusion range ,there is no obvious difference in 2D group when comparing after viewing to before,however,a statistically signif icant difference exists in 3D group.Both groups have significant differences in AC/A and CFF before and after viewing.Compared with the 2D group,3D group has a much more significantly decrease towards the three indicat ors during viewing times.The experimental results show that 3D material causes more severe visual fatigue and VIMS than 2D counterpart, while motion sickness is the main cause of discomfort in viewing autostereoscopi c 3D display.