Hemoglobin is an indispensable protein of the human body,and its cont ent is an important physiological index reflecting the health status of human blood.In order to mon itor human hemoglobin concentration accurately and in real time,an interventional hemoglobin concentr ation monitoring system is established in this paper.The human hemoglobin content is measured using a mini mally invasive method.In order to improve the accuracy of the measurement results,correction coefficients are introduced for the voltage collection of the two photodetectors respectively: α₁＝0．934 ,α₂＝0．956,and the corrected voltage values are more uniform and stable.The blood samples of 10heterosexual subject s were measured for 10consecutive times,and the largest mean square error of the test results was σ＝0．8390,indicating that the hemoglobin concentration monitoring system has good stability.A single measurem ent of another 50subjects was a group of samples.Based on the data analysis of the measured value and the rea l value detected by the hospital, the correlation coefficient of the sample of the male subject is R ＝0．8874,and the correlation coefficient of the sample of the female subject is R＝0．9002.The measurement result has high reliability and validity.The study of interventional determination of local hemoglobin concentration will play an i mportant guiding role in clinically minimally invasive surgery.