The molecular dipole moment orientatio n of the organic material can affect the output coupling efficiency of the flat OLED device,and has an important influence on the luminous efficiency of the OLE D.The 8-hydroxyquinoline aluminum (Alq₃) and 4,4-bis (9-carbazole) biphenyl with different molecular configurations prepared by the pull-up film forming m ethod were analyzed by measuring the polarization spectrum and Gaussian software fitting.The experimental results and theoretical calculation analysis showed th at the Alq_3molecule has an intrinsic dipole moment of 5.7699Debye,while the intrinsic dipole moment of the CBP molecule is zero.The orie ntation of the two molecules are randomly distributed in the chloroform solution ,and have a certain degree of orientation along the pulling direction,showing an isotropy after pulling into film.The anisotropy of Alq3molecules in the film is greater than that in the solution,while the situation of CBP molecules is just the opposite.We think that molecules with or without intrinsic dipole moment and the dipole moment orientation have a great effect on the different polarization absorption and fluorescence phenomena.