The Radix Notoginseng has huge consumption as raw material in modern C hinese patent medicine market because of its important medicinal value,therefore,the study of Radix N otoginseng has practical significance.Radix Notoginseng powder contains dozens of chemical constituents, which is a typical multi-component substance and difficult to quantitatively analyze by traditiona l two-dimensional fluorescence spectroscopy.Three-dimensional synchronous fluorescence of Radix Notoginseng p owder of 22solution samples were measured by using FLS920spectrometer.Combined with Second-Order Calibrat ion,Split half Method and Core Consistency Diagnostic confirm that the number of the components was 3in R adix Notoginseng powder solution.The first component synchronous fluorescence peak in this region λEx=380~390nm,λEm=465~ 475nm;the second component synchronous fluorescence peak in this region λEx=465~475nm,λEm=515~525nm;the th ird component synchronous fluorescence peak in this region λEx=330~340nm,λEm=415~425nm.The fluoresc ence characteristic of third component may be the fluorescence of Ginsenoside R_g1.Experimental works lead to the conclusion that three-dimensional synchronous fluorescence method combined with Second-Order Calibration can simplify the difficulty of spectral analysis,when multiple components coexist a nd there are many interfering substances.The study can provide the reference for analysis the multi-componen t of natural medicine.